The specificity of plastoquinone as a cofactor for photophosphorylation.

نویسندگان

  • D W KROGMANN
  • E OLIVERO
چکیده

The requirement for plastoquinone in the photosynthetic phosphorylation activity of spinach chloroplasts has been indicated in an earlier publication (1). Removal of endogenous plastoquinone from lyophilized chloroplasts by heptane extraction caused a loss of photophosphorylation activity. The photophosphorylation activity could be partially restored by addition of pure plastoquinone to the extracted preparations. The Hill reaction activity with 2,3’, 6trichlorophenol indophenol responded in a fashion similar to photophosphorylation with these preparations. It was shown that this restoration of activities could be made proportional to the concentration of plastoquinone available to the heptane-extracted chloroplasts. This paper reports in detail the techniques used in such experiments and the specificity of plastoquinone as a cofactor for photophosphorylation.

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عنوان ژورنال:
  • The Journal of biological chemistry

دوره 237  شماره 

صفحات  -

تاریخ انتشار 1962